Marble Burying Test Autism

The marble burying test is a useful model of both obsessive compulsive like ocd behavior and or anxiety like behavior.

Marble burying test autism.

The marble burying test in animal models of asd such as the btbr t tf j mouse eif4e transgenic mice 4e tg as well as in the maternal immune activation mouse model amodeo et al 2012. Santini et al 2013. In the current study significant differences in marble burying behavior were observed between pm 2 5 exposed and control rats figure 1b and supplementary figure 3 f 3 35 37 56 p 001. Choi et al 2016.

The marble burying test protocol described below is modified from deacon 2006 and angoa perez kane. Marble burying test and self grooming behavior. The marble burying test is commonly used to detect repetitive digging behavior servadio et al 2015. Burrows escape tunnels and in standard cage bedding and the nestlet shred test capitalizes on the fact that mice are nesting animals by nature that build nests for protection of themselves and their offspring against environmental conditions e g.

For the repetitive domain the t maze spontaneous alternation test and marble burying test are described. The setup for the marble burying test consists of a standard mouse housing cage filled with a thick layer 5 cm of shaved aspen bedding. In this unit behavioral tests are described that are relevant to the domains affected in asd. Subjects received a different treatment before marble burying from that administered during the spontaneous self grooming experiment with the exception of three b6 mice.

The number of marbles that were buried by the mouse was measured. The marble burying test occurred 8 days following the grooming test to ensure there were no potential residual effects from the initial drug treatment birdsall et al 1978. While widely used there is significant controversy over the interpretation of its results. Marble burying is an animal model used in scientific research to depict anxiety or obsessive compulsive disorder ocd behavior.

Each mouse was placed for 45 min into a clean rat cage with 4 cm deep bedding and 12 glass marbles placed on the surface. If a transparent ceiling with breathing holes is placed on top of testing cage this will prevent the test subject from leaving the cage while allowing unrestricted video recording. It is based on the observation that rats and mice will bury either harmful or harmless objects in their bedding.